Chronic Diabetes mellitus (DM) is a metabolic disease with multiple etiologies. It is characterized by hyperglycemia resulting from defects in insulin secretion, action or both. The combined approach of human, in vitro and animal studies is probably the best strategy to improve our understanding of underlying mechanisms. Diabetic animal models are frequently used in the investigation and treatment of genetic and environmental factors in the emergence of DM and complications due to DM. In this study, we aimed to measure blood glucose levels in chick embryos by using different blood sampling methods and to find the most applicable blood sampling method. 50 specific pathogens free (SPF) fertilized chick eggs (White Leghorn chicken) were used in this study. 50 SPF eggs were used for blood sampling on 18th day of incubation. Blood sampling from SPF eggs were made using five different techniques on the 18th day of incubation. 10 SPF fertilized eggs were used for each technique. The stages of chick embryos were determined as stage 44 according to the Hamburger-Hamilton classification. Blood glucose level was measured in only three of the techniques (First, second and fourth technique). The technique with the highest mean blood glucose level (153.90±9.73) was determined as the fourth. The lowest mean was detected in the second technique (130.50±8.20). As a result, it is not possible to say that the three techniques are alternatives to each other. The second technique differs from other techniques according to the Bland-Altman method.