In the current study, it was examined the possible protective properties of hesperidin (HES), an antioxidant, antiautophagic, and antiapoptotic molecule, against ovarian injury caused by ischemia reperfusion. 24 healthy rats were grouped as the group I (sham), group II (ischemia reperfusion (I/R)) and group III (I/R+HES) (n=8). In the sham group, the only abdominal incision was performed and closed. In the I/R group, following the incision, the I/R model was carried out. In the I/R+HES group, HES was administered intraperitoneally to the animals at the dose of 100 mg/kg approximately 1 hour before reperfusion. All the experimental procedures were performed under anesthesia. Following reperfusion, ovarian tissues were removed, and then some antioxidant, oxidant, autophagic and apoptotic parameters were evaluated. The oxidant parameters elevated, and antioxidant parameters declined in the I/R group. On the other side, antioxidant parameters were raised and oxidant parameters diminished in the I/R+HES group. Besides, it was observed that Light chain 3 (LC3) B, caspase-3, and Nuclear factor-kappa Bp50 (NF-kBp50) immunopositivity were fairly intensive in the I/R group while it was less in the I/R+HES group. A single dose of HES is quite efficient against I/R-induced oxidative ovarian injury.