The aim of this study is to evaluate the cytotoxicity with agar diffusion test of two different universal adhesive systems with different content, pH, and polymerization techniques on mouse fibroblast cell lines (L929). G-Premio Bond (GC Europe, Belgium) and Tokuyama Universal Bond (Tokuyama, USA) were supplied from common universal adhesive systems. G-Premio Bond, which is light- polymerized, and Tokuyama Universal Bond adhesive systems, which are chemical-polymerized, are polymerized according to the manufacturer's instructions. All materials were incubated for 72 hours in Dulbecco's modified Eagle medium (DMEM) solution. L929 cells were placed in each wells to be 1.5x105 cells / ml and adhesive systems to be examined for cytotoxicity were applied. Waiting for the added agar to freeze, adhesive systems were installed and cell viability examined in an inverted microscope. Waiting for the added agar to freeze, adhesive systems were placed and cell viability examined in an inverted microscope. Statistical analysis was performed using one-way analysis of variance (ANOVA) and Tukey’s post-hoc test. A statistically significant difference was found between the two different Universal adhesive systems in terms of cell viability values. Cell viability was significantly lower in the Tokuyama Universal Bond group compared to the G-Premio Bond group (p <0.05). In treatments using universal adhesive systems, cytotoxic effects can be observed against live cells. The compatibility of the results should be supported by different cytotoxicity detection methods.
Cytotoxicity, adhesive system, agar diffusion test, L929 cells